The genotoxicity of trenbolone, a synthetic steroid.
Huntingdon Research Centre, Cambs, England
Trenbolone, a synthetic androgen is used as a growth promotant in animal
husbandry. Because of its steroidal structure and properties it has been
extensively evaluated in a series of in vitro and in vivo assays to assess its
genotoxic and initiating properties. Both the parent molecule
17-beta-hydroxy-trenbolone and its metabolite 17-alpha-hydroxy-trenbolone,
produced only in cattle, have been tested.
17-beta-hydroxy-trenbolone was not genotoxic in the Ames Salmonella/microsome
assay, cytogenetics assays in human lymphocytes and CHO cells, a micronucleus
assay in CHO cells, a DNA repair synthesis assay in HeLa cells, mammalian cell
mutation assays with CHO and V79 cells, the mouse micronucleus assay, rat bone
marrow or spermatogonial cytogenetics assays or in a test for initiators in the
rat. In the mouse lymphoma cell mutation assay with L 5178Y TK+/- cells,
equivocal responses were obtained, particularly at highly toxic concentrations.
With 17-alpha-hydroxy-trenbolone a weak positive response was obtained in the
L5178Y Tk +/- assay, particularly at highly toxic concentrations. Negative
results were obtained in the Ames Salmonella/microsome assay, the cytogenetics
assays using both human lymphocytes in vitro and rat bone marrow in vivo, the
DNA repair assay and in the CHO mammalian cell mutation assay. It was also
negative in the in vivo test for initiators. From this extensive battery of
data, and also taking into account published data on trenbolone, it is concluded
that 17-alpha-hydroxytrenbolone and 17-beta-hydroxy-trenbolone are devoid of
genotoxic activity and are not initiators of cancer.
Arch Toxicol 1988;61(4):249-58 Related Articles, Books, LinkOut
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